T3405
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T3405
Sigma
3,3′,5,5′-Tetramethylbenzidine dihydrochloride
tablet, 1 mg substrate per tablet
| Synonym: | 4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride |
| CAS Number: | 64285-73-0 |
| Linear Formula: | C16H20N2 · 2HCl |
| Molecular Weight: | 313.27 |
| EC Number: | 264-769-6 |
| MDL number: | MFCD00012961 |
| PubChem Substance ID: | 24900140 |
Description
| Application | 3,3',5,5'-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. |
Properties
| form | tablet |
| mp | ≥300 °C |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
References
| reference | Holland, V.R., et al. Tet. 30, 3299, (1974) |
| Bos, E.S., et al. J. Immunoassay 2, 187, (1981) |
